MAP1LC3A Antikörper

Produktnummer ABIN388463

Produktdetails anti-MAP1LC3A Antikörper

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAP1LC3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This LC3 antibody is generated from rabbits immunized with full-length recombinant human LC3 (APG8a).

Klon: RB7993

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:200. WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-MAP1LC3A Antikörper (ABIN388463)

Want, Gillespie, Wang, Gordon, Iomini, Ritch, Wolosin, Bernstein: "Autophagy and Mitochondrial Dysfunction in Tenon Fibroblasts from Exfoliation Glaucoma Patients." in: PLoS ONE, Vol. 11, Issue 7, pp. e0157404, (2017) (PubMed).

Hu, Wu, Ding, Zhang: "Thrombin preferentially induces autophagy in glia cells in the rat central nervous system." in: Neuroscience letters, Vol. 630, pp. 53-58, (2017) (PubMed).

Wu, Hong, Wang, Huang, Yeh, Wang, Wang, Chiu: "A novel histone deacetylase inhibitor TMU-35435 enhances etoposide cytotoxicity through the proteasomal degradation of DNA-PKcs in triple-negative breast cancer." in: Cancer letters, Vol. 400, pp. 79-88, (2017) (PubMed).

Islam, Shin, Yun, Cho, Seo, Chae, Cho: "The effect of RNAi silencing of p62 using an osmotic polysorbitol transporter on autophagy and tumorigenesis in lungs of K-rasLA1 mice." in: Biomaterials, Vol. 35, Issue 5, pp. 1584-96, (2013) (PubMed).

Liu, Jia, Zhang, Hou, Wang, Hao, Ruan, Yu, Zheng: "Involvement of melatonin in autophagy-mediated mouse hepatoma H22 cell survival." in: International immunopharmacology, Vol. 12, Issue 2, pp. 394-401, (2012) (PubMed).

Zhang, Morgan, Chen, Choksi, Liu: "Induction of autophagy is essential for monocyte-macrophage differentiation." in: Blood, Vol. 119, Issue 12, pp. 2895-905, (2012) (PubMed).

Wojtkowiak, Sane, Kleinman, Sloane, Reiners, Mattingly: "Aborted autophagy and nonapoptotic death induced by farnesyl transferase inhibitor and lovastatin." in: The Journal of pharmacology and experimental therapeutics, Vol. 337, Issue 1, pp. 65-74, (2011) (PubMed).

Mizuno, Yasuo, Bogaard, Kraskauskas, Natarajan, Voelkel: "Inhibition of histone deacetylase causes emphysema." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 300, Issue 3, pp. L402-13, (2011) (PubMed).

Zheng, Liu, Li, Xu, Xu: "RNA interference-mediated downregulation of Beclin1 attenuates cerebral ischemic injury in rats." in: Acta pharmacologica Sinica, Vol. 30, Issue 7, pp. 919-27, (2009) (PubMed).

Details zu MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Andere Bezeichnung: LC3 (MAP1LC3A Produkte)

Hintergrund: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Gen-ID: 84557

NCBI Accession: NP_115903, NP_852610

UniProt: Q9H492, Q9GZQ8

Pathways: Autophagie