ATG12 Antikörper (N-Term)

Produktnummer ABIN388536

Produktdetails anti-ATG12 Antikörper

Target: ATG12 (Autophagy Related 12 (ATG12))

Bindungsspezifität: AA 1-30, N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG12 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATG12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ATG12.

Klon: RB7475

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:200. WB: 1:2000. IHC-P: 1:50~100. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-ATG12 Antikörper (ABIN388536)

Yang, Zhao, Mei, Jiang, Geng, Li, Yao, Liu, Kong, Cao: "HMGA2 plays an important role in Cr (VI)-induced autophagy." in: International journal of cancer, Vol. 141, Issue 5, pp. 986-997, (2017) (PubMed).

Chen, Cao, Zhou, Liu, Che, Mizumura, Li, Choi, Shen: "Interaction of caveolin-1 with ATG12-ATG5 system suppresses autophagy in lung epithelial cells." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 306, Issue 11, pp. L1016-25, (2014) (PubMed).

Sanchez, Penfornis, Oskowitz, Boonjindasup, Cai, Dhule, Rowan, Kelekar, Krause, Pochampally: "Activation of autophagy in mesenchymal stem cells provides tumor stromal support." in: Carcinogenesis, Vol. 32, Issue 7, pp. 964-72, (2011) (PubMed).

Yee, Wilkinson, James, Ryan, Vousden: "PUMA- and Bax-induced autophagy contributes to apoptosis." in: Cell death and differentiation, Vol. 16, Issue 8, pp. 1135-45, (2009) (PubMed).

Harada, Willison, Sakakibara, Miyamoto, Fujita, Taniguchi: "Absence of the type I IFN system in EC cells: transcriptional activator (IRF-1) and repressor (IRF-2) genes are developmentally regulated." in: Cell, Vol. 63, Issue 2, pp. 303-12, (1990) (PubMed).

Details zu ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Andere Bezeichnung: ATG12 (ATG12 Produkte)

Hintergrund: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG12L is the human homolog of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy.

Molekulargewicht: 15113

Gen-ID: 9140

NCBI Accession: NP_001264712, NP_004698

UniProt: O94817

Pathways: Autophagie