LGR5 Antikörper (AA 689-719)

Produktnummer ABIN389218

Produktdetails anti-LGR5 Antikörper

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Bindungsspezifität: AA 689-719

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LGR5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This LGR5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 689-719 amino acids from human LGR5.

Klon: RB14211

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. IF: 1:100. FC: 1:25

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Haltbarkeit: 6 months

Referenzen für anti-LGR5 Antikörper (ABIN389218)

Huch, Gehart, van Boxtel, Hamer, Blokzijl, Verstegen, Ellis, van Wenum, Fuchs, de Ligt, van de Wetering, Sasaki, Boers, Kemperman, de Jonge, Ijzermans, Nieuwenhuis, Hoekstra, Strom, Vries et al.: "Long-term culture of genome-stable bipotent stem cells from adult human liver. ..." in: Cell, Vol. 160, Issue 1-2, pp. 299-312, (2015) (PubMed).

Ying, Tsujii, Kondo, Hayashi, Kato, Akasaka, Inoue, Shiraishi, Inoue, Hiyama, Tsujii, Maekawa, Kawai, Fujinaga, Araki, Shinzaki, Watabe, Nishida, Iijima, Takehara: "The effectiveness of an anti-human IL-6 receptor monoclonal antibody combined with chemotherapy to target colon cancer stem-like cells." in: International journal of oncology, Vol. 46, Issue 4, pp. 1551-9, (2015) (PubMed).

Singh, Mallela, Cornuet, Reifler, Chervenak, West, Wong, Nasonkin: "Characterization of Three-Dimensional Retinal Tissue Derived from Human Embryonic Stem Cells in Adherent Monolayer Cultures." in: Stem cells and development, Vol. 24, Issue 23, pp. 2778-95, (2015) (PubMed).

Jardé, Kass, Staples, Lescesen, Carne, Oliva, McMurrick, Abud: "ERBB3 Positively Correlates with Intestinal Stem Cell Markers but Marks a Distinct Non Proliferative Cell Population in Colorectal Cancer." in: PLoS ONE, Vol. 10, Issue 9, pp. e0138336, (2015) (PubMed).

Bu, Zheng, Zhang, Li, Sun, Dong, Wu, Wu, Wang, Cheng, Xing, Li, Du, Ji: "LGR5 is a promising biomarker for patients with stage I and II gastric cancer." in: Chinese journal of cancer research = Chung-kuo yen cheng yen chiu, Vol. 25, Issue 1, pp. 79-89, (2013) (PubMed).

Zheng, Sun, Bu, Zhang, Li, Wu, Wu, Wang, Cheng, Xing, Du, Ji: "Intestinal stem cell marker LGR5 expression during gastric carcinogenesis." in: World journal of gastroenterology : WJG, Vol. 19, Issue 46, pp. 8714-21, (2013) (PubMed).

Maddox, Shakya, South, Shelton, Andersen, Chidester, Kang, Gligorich, Jones, Spangrude, Welm, Tantin: "Transcription factor Oct1 is a somatic and cancer stem cell determinant." in: PLoS genetics, Vol. 8, Issue 11, pp. e1003048, (2012) (PubMed).

Judah, Rudkouskaya, Wilson, Carter, Dagnino: "Multiple roles of integrin-linked kinase in epidermal development, maturation and pigmentation revealed by molecular profiling." in: PLoS ONE, Vol. 7, Issue 5, pp. e36704, (2012) (PubMed).

Takahashi, Ishii, Nishida, Takemasa, Mizushima, Ikeda, Yokobori, Mimori, Yamamoto, Sekimoto, Doki, Mori: "Significance of Lgr5(+ve) cancer stem cells in the colon and rectum." in: Annals of surgical oncology, Vol. 18, Issue 4, pp. 1166-74, (2011) (PubMed).

Satoh, Obayashi, Tabunoki, Wakana, Kim: "Stable expression of neurogenin 1 induces LGR5, a novel stem cell marker, in an immortalized human neural stem cell line HB1.F3." in: Cellular and molecular neurobiology, Vol. 30, Issue 3, pp. 415-26, (2010) (PubMed).

Details zu LGR5

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Andere Bezeichnung: LGR5/GPR49 (LGR5 Produkte)

Synonyme: lgr5-a antikoerper, lgr5a antikoerper, FEX antikoerper, Gpr49 antikoerper, GPR49 antikoerper, GPR67 antikoerper, GRP49 antikoerper, HG38 antikoerper, leucine-rich repeat containing G protein-coupled receptor 5 L homeolog antikoerper, leucine rich repeat containing G protein coupled receptor 5 antikoerper, leucine rich repeat containing G protein-coupled receptor 5 antikoerper, lgr5.L antikoerper, Lgr5 antikoerper, LGR5 antikoerper

Hintergrund: LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues. Stem cell marker of the intestinal epithelium and the hair follicle. Target gene of Wnt signaling. Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.

Molekulargewicht: 99998

Gen-ID: 8549

NCBI Accession: NP_001264155, NP_003658

UniProt: O75473

Pathways: WNT Signalweg