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SMAD3 Antikörper (pSer213)

Name: SMAD3 Antikörper (pSer213)
SKU: ABIN389657
Price: 467.5 EUR
Availability: InStock

SMAD3 Reaktivität: Human WB, IHC (p) Wirt: Kaninchen Polyclonal RB29526 unconjugated

The anti-Phospho-SD3- Pab (ABIN389657 and ABIN2839640) is used in Western blot to detect Phospho-SD3- in Ramos tissue lysate (SMAD3 Antikörper (pSer213))

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (SMAD3 Antikörper (pSer213))

IHC (p)

All lanes : Anti-SD3 Antibody at 1:1000 dilution Lane 1: HACAT whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: Hela whole cell lysate Lysates/proteins at 20 μg per lane. (SMAD3 Antikörper (pSer213))

3 Abbildungen

(1 reference)

Produktnummer ABIN389657

Datenblatt (PDF)

Target Alle SMAD3 Antikörper anzeigen

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
Bindungsspezifität
38

33

26

25

23

12

11

8

6

5

5

5

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer213
Reaktivität
227

153

135

27

9

9

8

7

7

4

3

3

2

2

1

1
Human
Wirt
219

22
Kaninchen
Passende Sekundärantikörper
Klonalität
204

37
Polyklonal
Konjugat
119

13

12

11

8

6

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

1

1

1

1
Dieser SMAD3 Antikörper ist unkonjugiert
Applikation
191

96

73

72

55

53

49

48

27

13

10

8

5

4

2

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Homologie

C, M, Pig, Rat

Aufreinigung

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This SMAD3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S213 of human SMAD3.

Klon

RB29526

Isotyp

Ig Fraction

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Applikationshinweise

WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit

6 months
Wu, Bian, Dou, Gong, Tan, Xia, Dai: "Asiaticoside hinders the invasive growth of keloid fibroblasts through inhibition of the GDF-9/MAPK/Smad pathway." in: Journal of biochemical and molecular toxicology, Vol. 31, Issue 8, (2017) (PubMed).
Target

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Andere Bezeichnung

SMAD3 (SMAD3 Produkte)

Hintergrund

SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80 % of all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes. SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes. Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma.

Molekulargewicht

48081

Gen-ID

4088

NCBI Accession

NP_001138574, NP_001138575, NP_001138576, NP_005893

UniProt

P84022

Pathways

Zellzyklus, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagie