DCLK1 Antikörper (AA 690-720)

Produktnummer ABIN391324

Produktdetails anti-DCLK1 Antikörper

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Bindungsspezifität: AA 690-720

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DCLK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This DCAMKL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-720 amino acids of human DCAMKL1.

Klon: RB3161

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:2000. WB: 1:1000. WB: 1:8000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Haltbarkeit: 6 months

Referenzen für anti-DCLK1 Antikörper (ABIN391324)

Kawamura, Takemoto, Nishimoto, Ueno, Hosoyama, Shirasawa, Tanaka, Kugimiya, Harada, Hamano: "Enhancement of cytotoxic effects of gemcitabine by Dclk1 inhibition through suppression of Chk1 phosphorylation in human pancreatic cancer cells." in: Oncology reports, Vol. 38, Issue 5, pp. 3238-3244, (2018) (PubMed).

Ushiama, Ishimaru, Narukawa, Yoshioka, Kozuka, Watanabe, Tsunoda, Osakabe, Asakura, Masuzaki, Abe: "Catecholamines Facilitate Fuel Expenditure and Protect Against Obesity via a Novel Network of the Gut-Brain Axis in Transcription Factor Skn-1-deficient Mice." in: EBioMedicine, Vol. 8, pp. 60-71, (2017) (PubMed).

Gao, Wang, Xu, Wen, Liu, An: "DCLK1 is up-regulated and associated with metastasis and prognosis in colorectal cancer." in: Journal of cancer research and clinical oncology, Vol. 142, Issue 10, pp. 2131-40, (2017) (PubMed).

Leppänen, Helminen, Huhta, Kauppila, Miinalainen, Ronkainen, Saarnio, Lehenkari, Karttunen: "Doublecortin-like kinase 1-positive enterocyte - a new cell type in human intestine." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 124, Issue 11, pp. 958-965, (2017) (PubMed).

Schellnegger, Quante, Rospleszcz, Schernhammer, Höhl, Tobiasch, Pastula, Brandtner, Abrams, Strauch, Schmid, Vieth, Wang, Quante: "Goblet Cell Ratio in Combination with Differentiation and Stem Cell Markers in Barrett Esophagus Allow Distinction of Patients with and without Esophageal Adenocarcinoma." in: Cancer prevention research (Philadelphia, Pa.), Vol. 10, Issue 1, pp. 55-66, (2017) (PubMed).

Nakata, Shimizu, Nagata, Ito, Fujii, Suzuki, Kawamoto, Ishibashi, Kuno, Anzai, Murano, Mizutani, Oshima, Tsuchiya, Nakamura, Hozumi, Watanabe, Okamoto: "Data showing proliferation and differentiation of intestinal epithelial cells under targeted depletion of Notch ligands in mouse intestine." in: Data in brief, Vol. 10, pp. 551-556, (2017) (PubMed).

Gross, Balderes, Liu, Asfaha, Gu, Wang, Sussel: "Nkx2.2 is expressed in a subset of enteroendocrine cells with expanded lineage potential." in: American journal of physiology. Gastrointestinal and liver physiology, pp. ajpgi.00244.2015, (2015) (PubMed).

Shimizu, Okamoto, Ito, Fujii, Nakata, Suzuki, Murano, Mizutani, Tsuchiya, Nakamura, Hozumi, Watanabe: "Distinct expression patterns of Notch ligands, Dll1 and Dll4, in normal and inflamed mice intestine." in: PeerJ, Vol. 2, pp. e370, (2014) (PubMed).

Gonzalez, Williamson, Piedrahita, Blikslager, Magness: "Cell lineage identification and stem cell culture in a porcine model for the study of intestinal epithelial regeneration." in: PLoS ONE, Vol. 8, Issue 6, pp. e66465, (2013) (PubMed).

Trivedi, Wiber, El-Zimaity, Brubaker: "Glucagon-like peptide-2 increases dysplasia in rodent models of colon cancer." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 302, Issue 8, pp. G840-9, (2012) (PubMed).

Yu, Chen, Zhang, Li, Xu, Wang, Ai, Liu: "Krüppel-like factor 4 regulates intestinal epithelial cell morphology and polarity." in: PLoS ONE, Vol. 7, Issue 2, pp. e32492, (2012) (PubMed).

Gerbe, van Es, Makrini, Brulin, Mellitzer, Robine, Romagnolo, Shroyer, Bourgaux, Pignodel, Clevers, Jay: "Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium." in: The Journal of cell biology, Vol. 192, Issue 5, pp. 767-80, (2011) (PubMed).

Gerbe, Brulin, Makrini, Legraverend, Jay: "DCAMKL-1 expression identifies Tuft cells rather than stem cells in the adult mouse intestinal epithelium." in: Gastroenterology, Vol. 137, Issue 6, pp. 2179-80; author reply 2180-1, (2009) (PubMed).

Dekaney, Gulati, Garrison, Helmrath, Henning: "Regeneration of intestinal stem/progenitor cells following doxorubicin treatment of mice." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 297, Issue 3, pp. G461-70, (2009) (PubMed).

May, Riehl, Hunt, Sureban, Anant, Houchen et al.: "Identification of a novel putative gastrointestinal stem cell and adenoma stem cell marker, doublecortin and CaM kinase-like-1, following radiation injury and in adenomatous polyposis coli/multiple ..." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 3, pp. 630-7, (2008) (PubMed).

Details zu DCLK1

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Andere Bezeichnung: DCAMKL1 (DCLK1 Produkte)

Synonyme: DCLK1 antikoerper, DCAMKL1 antikoerper, dcamkl1 antikoerper, dclk1 antikoerper, wu:fc51f11 antikoerper, zgc:153709 antikoerper, MGC145348 antikoerper, CL1 antikoerper, CLICK1 antikoerper, DCDC3A antikoerper, DCLK antikoerper, 1700113D08Rik antikoerper, 2810480F11Rik antikoerper, AI836758 antikoerper, Click-I antikoerper, Cpg16 antikoerper, Dcamkl1 antikoerper, Dcl antikoerper, Dclk antikoerper, mKIAA0369 antikoerper, Ania4 antikoerper, doublecortin like kinase 1 antikoerper, doublecortin-like kinase 1a antikoerper, serine/threonine-protein kinase DCLK1 antikoerper, doublecortin-like kinase 2 antikoerper, doublecortin-like kinase 1 antikoerper, DCLK1 antikoerper, dclk1a antikoerper, LOC587664 antikoerper, dclk2 antikoerper, LOC100539645 antikoerper, Dclk1 antikoerper

Hintergrund: Doublecortin-like kinase (DCAMKL1)(Ser/Thr protein kinase family) is essential for proper neurogenesis, neuronal migration, and axonal wiring. DCAMKL1 is involved in a calcium-signaling pathway controling neuronal migration in the developing brain, and participates in functions of the mature nervous system. DCAMKL1 protein shares high homology with doublecortin (DCX). DCLK, but not DCX, is highly expressed in regions of active neurogenesis in the neocortex and cerebellum. DCAMKL1 controls mitotic division by regulating spindle formation and also determines the fate of neural progenitors during cortical neurogenesis.

Molekulargewicht: 82224

Gen-ID: 9201

NCBI Accession: NP_001182344, NP_001182345, NP_004725

UniProt: O15075