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GAPDH Antikörper (C-Term)

Name: GAPDH Antikörper (C-Term)
SKU: ABIN392354
Price: 467.5 EUR
Availability: InStock

GAPDH Reaktivität: Human WB, IF, IHC (p), FACS Wirt: Kaninchen Polyclonal RB16543 unconjugated

Western blot analysis of GDH Antibody (C-term ) 7873b in , , CEM cell line lysates (35 μg/lane). (GAPDH Antikörper (C-Term))

Flow cytometric analysis of HepG2 cells using GDH Antibody (C-term )(bottom histogram) compared to a negative control cell (top histogram). (GAPDH Antikörper (C-Term))

FACS

Confocal immunofluorescent analysis of GDH Antibody (C-term ) 7873b with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (GAPDH Antikörper (C-Term))

All lanes : Anti-GDH Antibody (C-term ) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane. (GAPDH Antikörper (C-Term))

Western blot analysis of lysates from Hela,HUVEC cell line (from left to right),using GDH Antibody (C-term ) 7873b. (GAPDH Antikörper (C-Term))

Formalin-fixed and paraffin-embedded human prostate carcinoma with GDH Antibody (C-term ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (GAPDH Antikörper (C-Term))

IHC (p)

7 Abbildungen

(1 reference)

Produktnummer ABIN392354

Datenblatt (PDF)

Target Alle GAPDH Antikörper anzeigen

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
Bindungsspezifität
33

13

11

7

6

6

6

5

4

4

3

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 233-259, C-Term
Reaktivität
193

129

112

49

44

38

24

24

22

19

19

19

17

15

14

14

10

9

8

6

4

4

4

4

4

4

4

3

3

2

2

2

2

1

1

1

1

1

1
Human
Wirt
145

82

16

11

4
Kaninchen
Passende Sekundärantikörper
Klonalität
168

88
Polyklonal
Konjugat
152

30

16

15

3

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser GAPDH Antikörper ist unkonjugiert
Applikation
202

98

73

65

35

34

29

26

15

14

7

6

5

4

3

3

3

3

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Homologie

C, M, Pig, Rat

Aufreinigung

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This GAPDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-259 amino acids from the C-terminal region of human GAPDH.

Klon

RB16543

Isotyp

Ig Fraction

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GAPDH Reaktivität: Human, Maus, Ratte WB, IF, IHC (p) Wirt: Maus Monoclonal 1A10A10 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH Reaktivität: Human, Ratte WB, ELISA, IHC, IF Wirt: Ziege Polyclonal unconjugated

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GAPDH Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF Wirt: Ziege Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH Reaktivität: Human, Maus, Ratte WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
GAPDH Reaktivität: Human WB, IF, IHC (p) Wirt: Kaninchen Polyclonal RB16537 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibody
GAPDH Reaktivität: Human WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibody
GAPDH Reaktivität: Human WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

IF: 1:10~50. IF: 1:10~50. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit

6 months
Wang, Chen, Wang, Yu, Zhao, Zhang, Tang, Peng: "Metalloproteases meprin-ɑ (MEP1A) is a prognostic biomarker and promotes proliferation and invasion of colorectal cancer." in: BMC cancer, Vol. 16, pp. 383, (2017) (PubMed).
Target

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung

GAPDH (GAPDH Produkte)

Hintergrund

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

Molekulargewicht

36053

Gen-ID

2597

NCBI Accession

NP_001243728, NP_002037

UniProt

P04406