Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Homologie
B
Aufreinigung
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This MST1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-415 amino acids from the C-terminal region of human MST1.
MST1
Reaktivität: Human
IHC, ELISA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Haltbarkeit
6 months
Attarha, Andersson, Mints, Souchelnytskyi: "Individualised proteome profiling of human endometrial tumours improves detection of new prognostic markers." in: British journal of cancer, Vol. 109, Issue 3, pp. 704-13, (2013) (PubMed).
Ren, Yan, You, Sun: "Down-regulation of mammalian sterile 20-like kinase 1 by heat shock protein 70 mediates cisplatin resistance in prostate cancer cells." in: Cancer research, Vol. 68, Issue 7, pp. 2266-74, (2008) (PubMed).
MST1 is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it is possible that this protein induces the chromatin condensation observed in this process.