Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Homologie
Rat
Aufreinigung
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This SPAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-376 amino acids from the Central region of human SPAK.
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Haltbarkeit
6 months
Yang, Cai, Zhou, Chen, Chen, Chen, Wang, Fang, Zhou: "STE20/SPS1-related proline/alanine-rich kinase is involved in plasticity of GABA signaling function in a mouse model of acquired epilepsy." in: PLoS ONE, Vol. 8, Issue 9, pp. e74614, (2013) (PubMed).
Wang, OConnell, McArdle, Wade, Dorff, Shah, Shi, Pan, Rampersaud, Shen, Kim, Subramanya, Steinle, Parsa, Ober, Welling, Chakravarti, Weder, Cooper, Mitchell, Shuldiner, Chang: "From the Cover: Whole-genome association study identifies STK39 as a hypertension susceptibility gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 1, pp. 226-31, (2009) (PubMed).
Smith, Smallwood, Altman, Liedtke: "PKCdelta acts upstream of SPAK in the activation of NKCC1 by hyperosmotic stress in human airway epithelial cells." in: The Journal of biological chemistry, Vol. 283, Issue 32, pp. 22147-56, (2008) (PubMed).
Liedtke, Wang, Smallwood: "Role for protein phosphatase 2A in the regulation of Calu-3 epithelial Na+-K+-2Cl-, type 1 co-transport function." in: The Journal of biological chemistry, Vol. 280, Issue 27, pp. 25491-8, (2005) (PubMed).
SPAK is a serine/threonine kinase containing an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain, a nuclear localization signal, a consensus caspase cleavage recognition motif, and a C-terminal region. Northern blot analysis detects ubiquitous expression, most abundantly in brain and pancreas. SPAK can phosphorylate itself and an exogenous substrate in vitro. SPAK immunoprecipitates from transfected mammalian cells in a complex with another serine/threonine kinase that phosphorylates catalytically inactive SPAK. SPAK activates the p38 MAP kinase pathway in cotransfection assays. Full-length SPAK is expressed in the cytoplasm in transfected cells, while a mutant corresponding to caspase-cleaved STK39 localizes predominantly in the nucleus.