PIK3C3 Antikörper (N-Term)

Produktnummer ABIN392555

Produktdetails anti-PIK3C3 Antikörper

Target: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Bindungsspezifität: AA 24-53, N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PIK3C3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Homologie: M, Pig, Rat, X

Aufreinigung: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human PI3KC3.

Klon: RB1695

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:10~50. WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-PIK3C3 Antikörper (ABIN392555)

Wang, Li, Du, Xu, Wang, Zhang, Xu, Zeng, Mao, Cao: "The Class I PI3K inhibitor S14161 induces autophagy in malignant blood cells by modulating the Beclin 1/Vps34 complex." in: Journal of pharmacological sciences, Vol. 134, Issue 4, pp. 197-202, (2018) (PubMed).

Won, Yen, Lin, Jiang-Shieh, Lin, Chen, Su: "Autophagy mediates cytotoxicity of human colorectal cancer cells treated with garcinielliptone FC." in: Journal of cellular physiology, Vol. 233, Issue 1, pp. 497-505, (2017) (PubMed).

Lin, Wang, Hsu, Cho, Yang, Chien: "Capsaicin Induces Autophagy and Apoptosis in Human Nasopharyngeal Carcinoma Cells by Downregulating the PI3K/AKT/mTOR Pathway." in: International journal of molecular sciences, Vol. 18, Issue 7, (2017) (PubMed).

Zhang, Morgan, Chen, Choksi, Liu: "Induction of autophagy is essential for monocyte-macrophage differentiation." in: Blood, Vol. 119, Issue 12, pp. 2895-905, (2012) (PubMed).

McLeod, Zhou, Li, Wang, He: "The class III kinase Vps34 promotes T lymphocyte survival through regulating IL-7R? surface expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 10, pp. 5051-61, (2011) (PubMed).

Zhou, Wang, Hasegawa, Amin, Han, Kaneko, He, Wang: "Deletion of PIK3C3/Vps34 in sensory neurons causes rapid neurodegeneration by disrupting the endosomal but not the autophagic pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 20, pp. 9424-9, (2010) (PubMed).

Wang, Whiteman, Lian, Wang, Singh, Huang, Denmark: "A non-canonical MEK/ERK signaling pathway regulates autophagy via regulating Beclin 1." in: The Journal of biological chemistry, Vol. 284, Issue 32, pp. 21412-24, (2009) (PubMed).

Miselli, Negri, Gronchi, Losa, Conca, Brich, Fumagalli, Fiore, Casali, Pierotti, Tamborini, Pilotti: "Is autophagy rather than apoptosis the regression driver in imatinib-treated gastrointestinal stromal tumors?" in: Translational oncology, Vol. 1, Issue 4, pp. 177-86, (2008) (PubMed).

Liang, Feng, Ku, Dotan, Canaani, Oh, Jung: "Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG." in: Nature cell biology, Vol. 8, Issue 7, pp. 688-99, (2006) (PubMed).

Details zu PIK3C3

Target: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Andere Bezeichnung: PI3KC3 (PIK3C3 Produkte)

Synonyme: im:7147158 antikoerper, wu:fc13g06 antikoerper, wu:fz51e12 antikoerper, zgc:112016 antikoerper, zgc:113009 antikoerper, VPS34 antikoerper, hVps34 antikoerper, 5330434F23Rik antikoerper, Vps34 antikoerper, phosphatidylinositol 3-kinase catalytic subunit type 3 antikoerper, phosphatidylinositol 3-kinase catalytic subunit type 3 L homeolog antikoerper, phosphatidylinositol 3-kinase, catalytic subunit type 3 antikoerper, PIK3C3 antikoerper, pik3c3.L antikoerper, pik3c3 antikoerper, Pik3c3 antikoerper

Hintergrund: PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

Molekulargewicht: 101549

Gen-ID: 5289

NCBI Accession: NP_002638

UniProt: Q8NEB9

Pathways: AMPK Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Toll-Like Receptors Cascades, Autophagie