Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Homologie
B, Pig, Rat
Aufreinigung
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This UCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-48 amino acids from the N-terminal region of human UCK.
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Haltbarkeit
6 months
Zlatopolskiy, Morgenroth, Kunkel, Urusova, Dinger, Kull, Lepping, Reske: "Synthesis and biologic study of IV-14, a new ribonucleoside radiotracer for tumor visualization." in: Journal of nuclear medicine : official publication, Society of Nuclear Medicine, Vol. 50, Issue 11, pp. 1895-903, (2009) (PubMed).
UCK1 (Uridine-cytidine kinase 1) phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. This enzyme does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Of note, UCK1 is able to use either ATP or GTP as a phosphate donor. UCK1 also possesses the ability to phosphorylate a number of cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine.