HBxAg Antikörper

Produktnummer ABIN393198

Produktdetails anti-HBxAg Antikörper

Target: HBxAg (Hepatitis B Virus X Antigen (HBxAg))

Reaktivität: Hepatitis B Virus (HBV)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HBxAg Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)

Spezifität: Recognizes the HB-X antigen of hepatitis virus. The Hepatitis B X antigen is a 154 amino acid ~17 kD multifunctional protein involved in the development of liver cirrhosis and hepatocellular carcinoma. Clone 3F6-G10 was produced by immunization of mice with a "HB-X- Protein A" fusion construct and subsequent screening of hybridoma products against a "HB-X-GST" fusion construct. Has been used successfully for the detection of the hepatitis B X antigen by immunohistochemistry in formalin fixed, paraffin embedded material, also by western blotting against the immunizing and screening fusion proteins. Subsequently clone 3F6-G10 has been used as a capture reagent in a sensitive sandwich ELISA and bead based flow assay for the quantitative assessment of HbX antigen in human sera. Fine epitope mapping by phage library screening indicates that the epitope recognized bylies between amino acids 88 and 93 of the X antigen, a result subsequently confirmed by peptide ELISA.

Aufreinigung: Affinity purified

Immunogen: HB-Xag-Protein A Fusion protein.

Type of Immunogen: Fusion protein

Klon: 3F6-G10

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-Fr (1:100), IHC-P (1:100), WB (1:100 - 1:500)

Usage: Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

Kommentare:

Target Species of Antibody: Hepatitis B Virus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 0.1 % BSA.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Store undiluted.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Details zu HBxAg

Target: HBxAg (Hepatitis B Virus X Antigen (HBxAg))

Andere Bezeichnung: Hepatitis B X Antigen (HBxAg) (HBxAg Produkte)

Substanzklasse: Viral Protein