P4HB Antikörper (AA 499-509)

Produktnummer ABIN396933

Produktdetails anti-P4HB Antikörper

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Bindungsspezifität: AA 499-509

Reaktivität: Human, Maus, Ratte, Hamster, Rind (Kuh), Hund, Meerschweinchen, Affe, Schwein, Schaf, Kaninchen, Xenopus laevis, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser P4HB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Recognizes rat protein disulfide isomerase at ~58kD.

Aufreinigung: Protein G purified

Immunogen: Synthetic peptide corresponding to aa499-509 of rat protein disulfide isomerase (KLH coupled).

Type of Immunogen: Synthetic peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: HeLa Heat Shocked Cell Lysate, MCF-7 cell lysate.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Andere Bezeichnung: PDI / P4HB (P4HB Produkte)

Hintergrund: Name/Gene ID: P4HB

Synonyms: P4HB, ERBA2L, DSI, GIT, p4HB, p4Hbeta, PDIA1, PHDB, PO4DB, PROHB, Protocollagen hydroxylase, Protein disulfide-isomerase, PO4HB, PDI

Gen-ID: 5034

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism