CRE Recombinase (CRE) Antikörper

Produktnummer ABIN398566

Produktdetails

Target: CRE Recombinase (CRE)

Reaktivität: Bacteriophage P1

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Negative Control: E. coli. and cell lysate (not mammalian) It's not suitable for the analysis of mammalian cell lysate.

Kreuzreaktivität (Details): Negative Control: E. coli. and cell lysate (not mammalian) It's not suitable for the analysis of mammalian cell lysate.

Aufreinigung: Protein A affinity column

Immunogen: Purified recombinant Cre recombinase protein

Klon: 1D8H3

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The chart below may serve as a useful guideline, but secondary antibody affinity, antigen concentration, temperature, incubation time, and the sensitivity of the detection methods can all affect the ideal working concentration. We have not yet determined the suitability of this antibody for other applications. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL Western blot: 0.1-1.0 µg/mL Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen

Lin, Liu, Sun, Yuan, Zhang, Chen: "Establishment and characterization of a tamoxifen-mediated reversible immortalized mouse dental papilla cell line." in: In vitro cellular & developmental biology. Animal, Vol. 49, Issue 2, pp. 114-21, (2013) (PubMed).

Kaushik, Arias, Kwon, Lopez, Athonvarangkul, Sahu, Schwartz, Pessin, Singh: "Loss of autophagy in hypothalamic POMC neurons impairs lipolysis." in: EMBO reports, Vol. 13, Issue 3, pp. 258-65, (2012) (PubMed).

Antigendetails

Target: CRE Recombinase (CRE)

Andere Bezeichnung: Cre Recombinase (CRE Produkte)

Synonyme: cyclization recombinase antikoerper, cre antikoerper

Substanzklasse: Phage Protein

Hintergrund: Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific to a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the phage integrase family.Mouse Anti-Cre recombinase Monoclonal Antibody is an important tool for the identification of Cre recombinase in different protein expression systems.