Goat Anti-GST-tag [HRP] Polyclonal Antibody detects GST fusion proteins in expression systems including bacterial expression system, mammalian expression system and others.
Aufreinigung
Immunoaffinity chromatography
Immunogen
Recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum.
GST
Reaktivität: Human
WB, ELISA
Wirt: Maus
Monoclonal
6G9C6
unconjugated
Applikationshinweise
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL Western blot: 0.2-1 µg/mLOther Applications: user-optimized
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Savar, Jahanian-Najafabadi, Mahdavi, Shokrgozar, Jafari, Bouzari et al.: "In silico and in vivo studies of truncated forms of flagellin (FliC) of enteroaggregative Escherichia coli fused to FimH from uropathogenic Escherichia coli as a vaccine candidate against urinary ..." in: Journal of biotechnology, Vol. 175, pp. 31-7, (2014) (PubMed).
Gish, Gagne, Han, Deyoung, Clark: "WUSCHEL-responsive At5g65480 interacts with CLAVATA components in vitro and in transient expression." in: PLoS ONE, Vol. 8, Issue 6, pp. e66345, (2013) (PubMed).
Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogeneity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished by using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by introducing site-specific proteases. Antibodies directed against GST can detect both the fusion protein during purification and the cleavage of GST from the protein of interest.Goat Anti-GST-tag [HRP] Polyclonal Antibody is developed in goat using a recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum. The antibody is highly purified by immunoaffinity chromatography from goat antiserum and is then conjugated to horseradish peroxidase.