ELISA. Flow Cytometry. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 1/50. This CD275/ICOS Ligand antibody was validated for use in immunohistochemistry on apanel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat inducedantigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slideswere incubated with biotinylated secondary antibody LS-D2, followed by alkalinephosphatase-streptavidin and chromogen. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! Shelf life: one year from despatch.
ICOS ligand (inducible T cell co stimulator ligand) is a member of the B7 family and the immunoglobulin superfamily. Human ICOS ligand is expressed by activated monocytes/macrophages and dendritic cells. It binds to a CD28 like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. This interaction plays an important role in the T cell costimulation pathway.Synonyms: B7 homolog 2, B7-like protein Gl50, B7-related protein 1, B7H2, B7RP-1, B7RP1, ICOSL, ICOSLG, KIAA0653