HRP Antikörper

Produktnummer ABIN400996

Produktdetails anti-HRP Antikörper

Target: HRP (Horseradish Peroxidase (HRP))

Reaktivität: Meerrettich

Wirt: Ratte

Klonalität: Polyklonal

Konjugat: Dieser HRP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB)

Immunogen: Peroxidase [Horseradish]

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/200 - 1/1000. Dot Blot. ELISA: 1/2000 - 1/6000. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 85.0 mg/mL (by Refractometry)

Buffer: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4

Konservierungsmittel: Azide free

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu HRP

Target: HRP (Horseradish Peroxidase (HRP))

Andere Bezeichnung: Horseradish Peroxidase / HRP (HRP Produkte)

Hintergrund: Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.