RNF34 Antikörper (N-Term)

Produktnummer ABIN401035

Produktdetails anti-RNF34 Antikörper

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Bindungsspezifität: AA 1-373, N-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Orang-Utan

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RNF34 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody is directed against RNF34 protein. Expect reactivity with isoform 1 and 2 of RNF34.

Kreuzreaktivität (Details): Species reactivity (expected):Orangutan, Bovine, Canine, Mouse, Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography on Protein A

Immunogen: Recombinant protein corresponding to amino acids 1-373 of human RNF34 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/4,000 - 1/20,000. Western Blot: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.1 mL of deionized water or equivalent.

Konzentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu RNF34

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Andere Bezeichnung: RFI / RNF34 (RNF34 Produkte)

Hintergrund: RNF34, also known as RING finger protein 34, RING finger protein RIFF, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, hRFI, Caspases-8 and -10-associated RING finger protein 1, CARP-1 and Caspase regulator CARP1, is a novel modulator of NF-kappaB activation. RNF34 possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RNF34 protects cells against apoptosis induced by TNF and may bind phosphatidylinositol phosphates. RNF34 is localized to both the nucleus and the cytoplasm. In the nucleus, a distinct speckled pattern is observed by immunofluorescence microscopy. In the cytoplasm, the protein is associated with membranes. RNF34 is expressed ubiquitously and can be detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. Multiple transcript variants have been reported for this protein.Synonyms: CARP-1, CARP1, Caspases-8 and -10-associated RING finger protein 1, E3 ubiquitin-protein ligase RNF34, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, RIFF

Gen-ID: 80196

NCBI Accession: NP_079402

UniProt: Q969K3