TP53BP2 Antikörper

Produktnummer ABIN401045

Produktdetails anti-TP53BP2 Antikörper

Target: TP53BP2 (Tumor Protein P53 Binding Protein 2 (TP53BP2))

Reaktivität: Human, Hund, Makake

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TP53BP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody is directed against ASPP2.

Kreuzreaktivität (Details): Species reactivity (expected):Canine (100 %), Macaque (100 %), Mouse (80 %), Rat (80 %).
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Synthetic peptide corresponding to an internal sequence of Human ASPP2 . Genename: ASPP2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/2,500-1/10,000. Western Blot: 1/500-1/3,000. Expect a band approximately 125 kDa in size correspondingto ASPP2 in the appropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 2.4 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use.

Details zu TP53BP2

Target: TP53BP2 (Tumor Protein P53 Binding Protein 2 (TP53BP2))

Andere Bezeichnung: TP53BP2 (TP53BP2 Produkte)

Hintergrund: ASPP (ankyrinrepeat-, SH3-domain- proline-rich-region protein) proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, iASPP functionally inactivates p53. ASPPs may also regulate p63-and p73-mediated apoptosis. Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3. Not all cell cycle arrest genes are affected, such as p21. Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.Synonyms: 53BP2, ASPP2, Apoptosis-stimulating of p53 protein 2, BBP, Bcl2-binding protein, NY-REN-51, Tumor suppressor p53-binding protein 2, p53-binding protein 2, p53BP2

Gen-ID: 7159

NCBI Accession: NP_001026855

UniProt: Q13625