Biotin Antikörper (Rhodamine)

Produktnummer ABIN401178

Produktdetails anti-Biotin Antikörper (Rhodamine)

Target: Biotin

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Biotin Antikörper ist konjugiert mit Rhodamine

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Produktmerkmale: Absorption / Emission: 550 nm / 570 nm
Molar Ratio: 5.0 Moles TRITC per mole of Goat IgG

Aufreinigung: Affinity chromatography

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunofluorescence: 1/500 - 1/2,500. Flow Cytometry: 1/2,000 - 1/10,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 1.0 mL of deionized water (or equivalent).

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu Biotin

Target: Biotin

Abstract: Biotin Produkte

Substanzklasse: Chemical

Hintergrund: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.