HA-Tag Antikörper (Biotin)

Produktnummer ABIN401230

Produktdetails anti-HA-Tag Antikörper (Biotin)

Target: HA-Tag

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HA-Tag Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequenz: YPYDVPDYA

Produktmerkmale: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule

Aufreinigung: Affinity chromatography

Immunogen: 9-aa epitope tag peptide YPYDVPDYA (11 114-122) from hemagglutinin influenza conjugated to KLH using maleimide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 15,000 - 1: 150,000. Immunohistochemistry on paraffin sections: 1: 250. Immunoprecipitation: 1: 250. Western blot: 1: 10,000 (In western blotting of bacterial extracts the antibody does notcross-react with endogenous proteins).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.1 mL of deionized water (or equivalent).

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. For extended storage add glycerol to 50%.

Details zu HA-Tag

Target: HA-Tag

Andere Bezeichnung: HA Epitope Tag (YPYDVPDYA) (HA-Tag Produkte)

Substanzklasse: Tag

Hintergrund: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.