MLF1IP Antikörper (Thr78)

Produktnummer ABIN401343

Produktdetails anti-MLF1IP Antikörper

Target: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Bindungsspezifität: Thr78

Reaktivität: Human, Rind (Kuh), Hund, Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MLF1IP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunofluorescence (IF)

Spezifität: This antibody is specific for MLF1IP protein phosphorylated at Thr78.

Kreuzreaktivität (Details): Species reactivity (expected):Canine, Bovine, Chimpanzee, Rat, Mouse.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids surrounding Thr78 of human MLF1IP protein. The immunogen peptide is phosphorylated at Thr78

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/5,000 - 1/25,000. Western Blot: 1/500 - 1/2,000. Immunocytochemistry: 1/100 - 1/500. Immunohistochemistry: 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.26 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu MLF1IP

Target: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Andere Bezeichnung: MLF1IP (MLF1IP Produkte)

Synonyme: MGC147270 antikoerper, CENP50 antikoerper, CENPU antikoerper, CENPU50 antikoerper, KLIP1 antikoerper, PBIP1 antikoerper, CENP-U antikoerper, CENP-50 antikoerper, 1700029A22Rik antikoerper, centromere protein U antikoerper, MLF1 interacting protein antikoerper, cenpu antikoerper, MLF1IP antikoerper, CENPU antikoerper, Cenpu antikoerper

Hintergrund: Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel pololike kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/ anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.Synonyms: CENP-U(50), CENPU, Centromere protein U, ICEN24, Interphase centromere complex protein 24, KLIP1, KSHV latent nuclear antigen-interacting protein 1, MLF1-interacting protein, PBIP, Polo-box-interacting protein 1

Gen-ID: 79682

NCBI Accession: NP_078905

UniProt: Q71F23