c-MET Antikörper (pTyr1349, pTyr1356)

Produktnummer ABIN401382

Produktdetails anti-c-MET Antikörper

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Bindungsspezifität: pTyr1349, pTyr1356

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser c-MET Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody is specific for c-Met protein phosphorylated at Y1349 and Y1356.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic peptide corresponding to residues surrounding Y1349 and Y1356 of human c-Met protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/450,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.47 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu c-MET

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Andere Bezeichnung: HGF Receptor (MET Produkte)

Synonyme: AUTS9 antikoerper, HGFR antikoerper, RCCP2 antikoerper, c-Met antikoerper, AI838057 antikoerper, HGF antikoerper, Par4 antikoerper, Hgfr antikoerper, c-met antikoerper, MET antikoerper, C-MET antikoerper, met antikoerper, met-A antikoerper, MET proto-oncogene, receptor tyrosine kinase antikoerper, met proto-oncogene antikoerper, MET proto-oncogene, receptor tyrosine kinase L homeolog antikoerper, MET antikoerper, Met antikoerper, met.L antikoerper

Hintergrund: C-Met is the receptor for hepatocyte growth factor (also known as scatter factor, HGF/SF), and belongs to the tyrosine kinase superfamily. Interaction of c-Met with HGF results in autophosphorylation of c-Met at multiple tyrosines. Phosphorylation of Y1234/1235 in the c-Met kinase domain is critical to kinase activation. When phosphorylated, Y1349 and Y1356, along with surrounding amino acids, form a unique bidentate docking site for substrates such as Gab1, Grb2, phosphatidylinositol 3-kinase (PI3K) and others. C-Met mainly uses the Gab1 scaffolding adaptor in its initial step of signal transmission. Well-characterized downstream signalling pathways that are activated by c-Met include the ERK/MAPK, PI3K-Akt/PKB, Crk-Rap and Rac-Pak pathways, resulting in proliferation and increased cell survival as well as complex motogenic and morphogenic cellular responses. Gene targeting in mice has allowed elucidation of some of the developmental roles of c-Met and HGF/SF, such as their essential function in the formation of skeletal muscle, placenta and liver. Many types of cancer exhibit sustained c-Met stimulation, overexpression, or mutation, including carcinomas of the colon, breast, ovary, lung, liver, prostate, thyroid and kidney, as well as melanomas and sarcomas. In addition to cancer studies, other research areas in which c-Met is under investigation include organogenesis, organ regeneration, angiogenesis and surgical wound healing.Synonyms: HGF/SF receptor, Hepatocyte growth factor receptor, MET, Met proto-oncogene tyrosine kinase, Scatter factor receptor, c-Met

Gen-ID: 4233

NCBI Accession: NP_000236

UniProt: P08581

Pathways: RTK Signalweg, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor