STAT5A Antikörper (Tyr694)

Produktnummer ABIN401413

Produktdetails anti-STAT5A Antikörper

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Bindungsspezifität: Tyr694

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser STAT5A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody is specific for Stat5alpha protein phosphorylated at Y694.

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Human.
Species reactivity (tested):Mouse.

Aufreinigung: Affinity chromatography

Immunogen: synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5alpha protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/25,000. Western Blot: 1/500 - 1/2,000. Immunohistochemistry: 20 - 40 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Andere Bezeichnung: STAT5 / STAT5A (STAT5A Produkte)

Hintergrund: Signal trans-ducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic trans-cription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5alpha and Stat5beta. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus.Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A

Gen-ID: 20850

NCBI Accession: NP_035618

UniProt: P42230

Pathways: JAK-STAT Signalweg, RTK Signalweg, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation