SAE1 Antikörper (Ser185)

Produktnummer ABIN401467

Produktdetails anti-SAE1 Antikörper

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Bindungsspezifität: Ser185

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SAE1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody is directed against SUMO Activating Enzyme E1 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Bovine, Canine, Chimpanzee, Mouse, Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic peptide corresponding to a region surrounding S185 of the human SUMO Activating Enzyme E1 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/100,000 - 1/125,000. Western Blot: 1/750 - 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.71 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu SAE1

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Andere Bezeichnung: SAE1 / AOS1 (SAE1 Produkte)

Hintergrund: SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) with SAE2 (also known as UBA2) forms a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitinlike SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1) subunit resembles the N-terminal half of yeast UBA1, the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.Synonyms: SUA1, SUMO-activating enzyme subunit 1, UBLE1A, Ubiquitin-like 1-activating enzyme E1A

Gen-ID: 10055

NCBI Accession: NP_005491

UniProt: Q9UBE0