Dieser FITC Antikörper ist konjugiert mit Alkaline Phosphatase (AP)
Applikation
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Dot Blot (DB)
Aufreinigung
Prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
ELISA: 1: 2,000 - 1: 12,000. Western blot: 1: 500 - 1: 2,500. Dot blot. Immunohistochemistry on frozen sections: 1: 200 - 1: 1,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA), IgG and Protease free, 0.01 % (w/v) Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
DO NOT FREEZE!!
Lagerung
4 °C
Informationen zur Lagerung
Store the antibody prior and following reconstitution (undiluted) at 2 - 8 °C.