CHEK1 Antikörper (pSer280)
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- Target Alle CHEK1 Antikörper anzeigen
- CHEK1 (Checkpoint Kinase 1 (CHEK1))
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Bindungsspezifität
- pSer280
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser CHEK1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB)
- Spezifität
- This antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 280.
- Aufreinigung
- Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Immunogen
- The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 280 (V-T-Sp-G-G).
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anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
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anti-Checkpoint Kinase 1 (CHEK1) (pSer286) antibodyCHEK1 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated
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anti-Checkpoint Kinase 1 (CHEK1) (pSer345) antibodyCHEK1 Reaktivität: Human WB, IF, IHC (p), ICC Wirt: Kaninchen Polyclonal unconjugated
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anti-Checkpoint Kinase 1 (CHEK1) antibodyCHEK1 Reaktivität: Human WB, ELISA, IHC, IF Wirt: Kaninchen Monoclonal 2F2 unconjugated Recombinant Antibody
anti-Checkpoint Kinase 1 (CHEK1) antibodyCHEK1 Reaktivität: Human, Maus WB, ELISA Wirt: Maus Monoclonal 2G1D5 unconjugated
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- Applikationshinweise
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Western Blot: 1/500approx. 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store the antibody (in aliquots) at-20 °C.
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- Target
- CHEK1 (Checkpoint Kinase 1 (CHEK1))
- Andere Bezeichnung
- CHK1 (CHEK1 Produkte)
- Hintergrund
- One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic dependent kinase Cdc2, which regulates entry into mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of Cdc2. It is hypothesized that Chk1 induces serine 216 phosphorylation of Cdc25C and subsequent 14-3-3 binding negatively regulated Cdc25C, thus preventing it from activating Cdc2.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1
- Gen-ID
- 1111
- NCBI Accession
- NP_001107593
- UniProt
- O14757
- Pathways
- p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur
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