This Monoclonal Antibody Ks 13.1 represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin. Polypeptide Reacting: Mr 54 000 polypeptide Human keratin K13 (formerly designated cytokeratin13, with minor affinity to keratins K14, Mr 50 000, and K16, Mr 48 000). Tumors Specifically Detected: Several squamous cell carcinomas, e.g. cervix carcinoma, transitional cell carcinoma of the bladder, craniopharyngioma. Tested Reactivities on Cultured on Cell Lines: Cell lines from squamous cell CA, e.g. A-431 from epidermoid CA of vulva, RT 112, RT-4 of urinary bladder.
Kreuzreaktivität (Details)
Species reactivity (tested):Human, Bovine, Rat.
Aufreinigung
Protein A Affinity Chromatography
Immunogen
Keratin K13 of Mr 54.000 purified from Human esophagus
Immunofluorescence. Immunohistochemistry on frozen and paraffin sections: Ready-to-use. Immunoblotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Cytokeratin 13 is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus.Synonyms: CK-13, CK13, K13, KRT-13, KRT13, Keratin type I cytoskeletal 13, Keratin-13