HCV NS3 Antikörper

Produktnummer ABIN452517

Produktdetails anti-HCV NS3 Antikörper

Target: HCV NS3 (Hepatitis C Virus NS3 (HCV NS3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HCV NS3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Produktmerkmale: Synonyms: Hepatitis C Virus Serine proteiase NS3

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: A highly antigenic polypeptide consisting of essential sequences of at least 60 residues inlength, which were selected from genes encoding the NS-3 region of Chinese HCV strains.

Klon: 24-08-14

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: Use of Hepatitis C Virus NS3 antibody (clone 24-8) at 4 μg/mL will allowvisualization of 200 ng/lane Synthetic NS-3 Protein under reducing and non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL

Buffer: 0.01 M PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu HCV NS3

Target: HCV NS3 (Hepatitis C Virus NS3 (HCV NS3))

Andere Bezeichnung: HCV NS3 (HCV NS3 Produkte)

Substanzklasse: Viral Protein

Hintergrund: HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins, because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.Synonyms: Hepatitis C Virus Serine proteiase NS3