Aflatoxin Antikörper

Produktnummer ABIN452582

Produktdetails anti-Aflatoxin Antikörper

Target: Aflatoxin (AFT)

Reaktivität: Aspergillus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Aflatoxin Antikörper ist unkonjugiert

Applikation: Lateral Flow (LF), Enzyme Immunoassay (EIA)

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Winstar rats were immunized with Aflatoxin M1-BSA conjugate.

Klon: 2-00E-004

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: ELISA: 100 ng of AFM1 can be detected as competitive conjugate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with double distillated water to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody in aliquots at -20 °C after reconstitution. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Aflatoxin

Target: Aflatoxin (AFT)

Andere Bezeichnung: Aflatoxin (AFT Produkte)

Substanzklasse: Chemical

Hintergrund: The aflatoxins are a group of closely related mycotoxins that are widely distributed in nature. The most important of the group is aflatoxin B1 (AFB1), which has a range of biological activities, including acute toxicity, teratogenicity, mutagenicity and carcinogenicity. In order for AFB1 to exert its effects, it must be converted to its reactive epoxide by the action of the mixed function mono-oxygenase enzyme systems (cytochrome P450-dependent) in the tissues (in particular, the liver) of the affected animal. This epoxide is highly reactive and can form derivatives with several cellular macromolecules, including DNA, RNA and protein. Cytochrome P450 enzymes may additionally catalyse the hydroxylation (to AFQ1 and AFM1) and demethylation (to AFP1) of the parent AFB1?Molecule, resulting in products less toxic than AFB1. Conjugation of AFB1 to glutathione (mediated by glutathione S-transferase) and its subsequent excretion is regarded as an important detoxification pathway in animals.