CILP Antikörper (Internal Region)

Produktnummer ABIN453982

Produktdetails anti-CILP Antikörper

Target: CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))

Bindungsspezifität: Internal Region

Reaktivität: Human, Ratte, Maus, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CILP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody recognizes CILP.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Mouse and Canine (Dog).
Species reactivity (tested):Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NYRRTDHEDPRVK, from the internal region of the protein sequence according to NP_003604.2.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Antibody Detection Limit Dilution: 1/128000. Western Blot: 0.1-0.3 μg/mL. Approx 150 kDa band observed in rat spinal cord lysates(calculated MW of 130 kDa according to human NP_003604.2 and of 130 kDa according toRat NP_001101631.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu CILP

Target: CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))

Andere Bezeichnung: CILP1 (CILP Produkte)

Hintergrund: CILP, Cartilage Intermediate Layer Protein, is specifically expressed in the articular cartilage intermediate layer (it cannot be found in the superficial nor in the deepest layers) and is involved in scaffolding. Overexpression of this protein may lead to impairment of chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage. Antibodies against CILP are detected in patients with early-stage knee osteoarthritis and defects in this gene may be a cause of susceptibility to lumbar disk disease.Synonyms: CILP-1, Cartilage intermediate layer protein 1, Cartilage intermediate-layer protein

Gen-ID: 8483

NCBI Accession: NP_003604

UniProt: O75339