Ly6g Antikörper

Produktnummer ABIN457407

Produktdetails anti-Ly6g Antikörper

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Ly6g Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: The rat monoclonal antibody RB6-8C5 detects Ly6G component of Gr-1 antigen, a commonly used surface marker of neutrophils.

Kreuzreaktivität (Details): Mouse

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Endotoxin-Niveau: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Murine granulocytes

Klon: RB6-8C5

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Functional application: Neutrophil depletion.
Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein.
Western blotting: Recommended dilution: 1 μg/mL.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Konservierungsmittel: Azide free

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-Ly6g Antikörper (ABIN457407)

Jung, Zindl, Lai, Weaver, Chaplin: "MMP induced by Gr-1+ cells are crucial for recruitment of Th cells into the airways." in: European journal of immunology, Vol. 39, Issue 8, pp. 2281-92, (2009) (PubMed).

dos Santos, Vaz Cardoso, Nascimento, Lino, Dorta, de Oliveira, Ribeiro-Dias: "Leishmania major: recruitment of Gr-1+ cells into draining lymph nodes during infection is important for early IL-12 and IFN gamma production." in: Experimental parasitology, Vol. 119, Issue 3, pp. 403-10, (2008) (PubMed).

Leendertse, Willems, Giebelen, Roelofs, Bonten, van der Poll: "Neutrophils are essential for rapid clearance of Enterococcus faecium in mice." in: Infection and immunity, Vol. 77, Issue 1, pp. 485-91, (2008) (PubMed).

Fan, Li, Levy, Fan, Hackam, Vodovotz, Yang, Tracey, Billiar, Wilson: "Hemorrhagic shock induces NAD(P)H oxidase activation in neutrophils: role of HMGB1-TLR4 signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 10, pp. 6573-80, (2007) (PubMed).

Koo, Gan: "The innate interferon gamma response of BALB/c and C57BL/6 mice to in vitro Burkholderia pseudomallei infection." in: BMC immunology, Vol. 7, pp. 19, (2006) (PubMed).

Nagendra, Schlueter: "Absence of cross-reactivity between murine Ly-6C and Ly-6G." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 58, Issue 2, pp. 195-200, (2004) (PubMed).

Brown, Blaho, Loiacono: "Susceptibility to experimental Lyme arthritis correlates with KC and monocyte chemoattractant protein-1 production in joints and requires neutrophil recruitment via CXCR2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 2, pp. 893-901, (2003) (PubMed).

Hestdal, Ruscetti, Ihle, Jacobsen, Dubois, Kopp, Longo, Keller: "Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 1, pp. 22-8, (1991) (PubMed).

Details zu Ly6g

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Andere Bezeichnung: Ly6G (Ly6g Produkte)

Synonyme: Gr-1 antikoerper, Gr1 antikoerper, Ly-6G antikoerper, lymphocyte antigen 6 complex, locus G antikoerper, Ly6g antikoerper

Hintergrund: Lymphocyte antigen 6 complex, locus G,Ly6G is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. Ly6G is a good marker for detection of peripheral neutrophils. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. Physiological role of Ly6G remains still unclear. Its treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.,Gr1, Gr-1, Ly-6G

Gen-ID: 546644

UniProt: A0A087WQF5