Actin Antikörper

Produktnummer ABIN457424

Produktdetails anti-Actin Antikörper

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reaktivität: Human, Maus, Ratte, Kaninchen, Huhn, Hund, Non-Human Primate, Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: The mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.

Kreuzreaktivität (Details): Human, Non-Human Primates, Mouse, Rat, Canine (Dog), Feline (Cat), Rabbit, Chicken

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: SDS extracted protein fraction of human myocardium

Klon: HHF35

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blotting: Recommended dilution: 1 μg/mL, positive control: murine femoral muscle, murine heart, negative control: HUVEC line, reducing conditions.
Immunohistochemistry (paraffin sections): Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-Actin Antikörper (ABIN457424)

Matsuo, Susumu, Tsutsumi, Azuma, Obata, Hayashi: "Glomus tumor of the omentum: a case report." in: Journal of surgical oncology, Vol. 96, Issue 7, pp. 633-6, (2007) (PubMed).

Matsuyama, Hisaoka, Hashimoto: "Angioleiomyoma: a clinicopathologic and immunohistochemical reappraisal with special reference to the correlation with myopericytoma." in: Human pathology, Vol. 38, Issue 4, pp. 645-51, (2007) (PubMed).

Ijzer, Roskams, Molenbeek, Ultee, Penning, Rothuizen, van den Ingh: "Morphological characterisation of portal myofibroblasts and hepatic stellate cells in the normal dog liver." in: Comparative hepatology, Vol. 5, pp. 7, (2006) (PubMed).

Handharyani, Ochiai, Iwata, Umemura: "Immunohistochemical and ultrastructural study of ito cells (fat-storing cells) in response to extrahepatic bile duct ligation in broiler chickens." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 63, Issue 5, pp. 547-52, (2001) (PubMed).

de Sousa, Schwarzschild, de Araújo, de Araújo: "Basal cell adenocarcinoma of the palate with squamous metaplasia." in: Journal of clinical pathology, Vol. 53, Issue 2, pp. 153-6, (2000) (PubMed).

Miettinen: "Antibody specific to muscle actins in the diagnosis and classification of soft tissue tumors." in: The American journal of pathology, Vol. 130, Issue 1, pp. 205-15, (1988) (PubMed).

Schmidt, Cone, Haas, Gown: "Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed against muscle actins." in: The American journal of pathology, Vol. 131, Issue 1, pp. 19-28, (1988) (PubMed).

Tsukada, McNutt, Ross, Gown: "HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues." in: The American journal of pathology, Vol. 127, Issue 2, pp. 389-402, (1987) (PubMed).

Tsukada, Tippens, Gordon, Ross, Gown: "HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization." in: The American journal of pathology, Vol. 126, Issue 1, pp. 51-60, (1987) (PubMed).

Details zu Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Andere Bezeichnung: Actin (muscle) (ACTA1 Produkte)

Synonyme: ACTA antikoerper, ASMA antikoerper, CFTD antikoerper, CFTD1 antikoerper, CFTDM antikoerper, MPFD antikoerper, NEM1 antikoerper, NEM2 antikoerper, NEM3 antikoerper, acta antikoerper, asma antikoerper, cftd antikoerper, cftd1 antikoerper, cftdm antikoerper, mpfd antikoerper, nem1 antikoerper, AA959943 antikoerper, Acta-2 antikoerper, Acts antikoerper, Actsk-1 antikoerper, actc antikoerper, actc1 antikoerper, cfk antikoerper, hm:zeh0631 antikoerper, zeh0631 antikoerper, acta1 antikoerper, hm:zeh0293 antikoerper, zgc:112098 antikoerper, actin, alpha 1, skeletal muscle antikoerper, actin, alpha 1, skeletal muscle L homeolog antikoerper, actin, alpha 1b, skeletal muscle antikoerper, actin, muscle antikoerper, actin, alpha skeletal muscle antikoerper, actin, alpha 1a, skeletal muscle antikoerper, ACTA1 antikoerper, acta1.L antikoerper, acta1 antikoerper, Acta1 antikoerper, acta1b antikoerper, LOC100533357 antikoerper, LOC109479779 antikoerper, LOC109108280 antikoerper, acta1a antikoerper

Hintergrund: Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.,Muscle specific actin

Pathways: Caspase Kaskade in der Apoptose, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development