Ovalbumin Antikörper (FITC)

Produktnummer ABIN457639

Produktdetails anti-Ovalbumin Antikörper (FITC)

Target: Ovalbumin (OVA)

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ovalbumin Antikörper ist konjugiert mit FITC

Applikation: Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Spezifität: Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Produktmerkmale: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to chicken ovalbumin

Aufreinigung: The IgG (7S) fraction is isolated and purified from hyperimmune antisera with strong precipitating activity and contains the bulk of the antibody specificity. It is free of other serum proteins as tested by immuno-electrophoresis and double radial immunodiffusion.

Immunogen: Highly purified egg albumin (MW 45,000) isolated from chicken egg whites. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: In immunocytochemical and immunohistochemical staining of ovalbumin at the cellular and subcellular level of appropriately treated cell and tissue substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80, depending on the method used.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG protein concentration 10 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.7. No foreign proteins added.

Buffer: FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by ad ding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Details zu Ovalbumin

Target: Ovalbumin (OVA)

Andere Bezeichnung: Ovalbumin (OVA Produkte)

Synonyme: 1600019A21Rik antikoerper, R86 antikoerper, SPI-CI antikoerper, Spi10 antikoerper, ovalbumin antikoerper, NK13 antikoerper, Spi12 antikoerper, BC052216 antikoerper, NK21 antikoerper, NK21L1 antikoerper, Spi13 antikoerper, NK26 antikoerper, Spi14 antikoerper, AW540195 antikoerper, AT2 antikoerper, Spi9 antikoerper, EID antikoerper, SPI3B antikoerper, Gm11396 antikoerper, OTTMUSG00000000720 antikoerper, SERPINB14 antikoerper, C76171 antikoerper, C76174 antikoerper, SPI3C antikoerper, SPIC antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 9b antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 6b antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 9f antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 9e antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 9d antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 6e antikoerper, ovalbumin (SERPINB14) antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 6c antikoerper, Serpinb9b antikoerper, Serpinb6b antikoerper, Serpinb9f antikoerper, Serpinb9e antikoerper, Serpinb9d antikoerper, Serpinb1-ps1 antikoerper, Serpinb6e antikoerper, OVAL antikoerper, Serpinb6c antikoerper

Hintergrund: A single precipitin line is obtained in immunoelectrophoresis against 1% chicken egg albumin in PBS. In double radial immunodiffusion of the antiserum against twofold dilutions of 1% chicken egg albumin in PBS and in the reverse arrangement of the chicken egg albumin solution against twofold dilutions of the antiserum, a singe precipitin line is obtained. The antiserum does not react with chicken serum