Fibrinogen Antikörper

Produktnummer ABIN458743

Produktdetails anti-Fibrinogen Antikörper

Target: Fibrinogen

Reaktivität: Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Fibrinogen Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: The antiserum does not cross-react with any other component of rat plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.

Produktmerkmale: Purified IgG fraction of polyclonal goat antiserum to rat fibrinogen

Aufreinigung: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: As unlabelled primary or secondary antibody reagent for the indirect detection of fibrinogen in rat cells, tissues and body fluids in immunofluorescence and immunoenzyme methods, for the production of immunoconjugates with a selected marker, to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier, as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Physicochemical characteristic IgG protein concentration 10 mg/ml. No foreign proteins added.

Buffer: Purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)

Konservierungsmittel: Without preservative

Lagerung: 4 °C

Referenzen für anti-Fibrinogen Antikörper (ABIN458743)

Chen, Schmidt, Schürger, Hankir, Krug, Rittner: "Netrin-1 as a Multitarget Barrier Stabilizer in the Peripheral Nerve after Injury." in: International journal of molecular sciences, Vol. 22, Issue 18, (2021) (PubMed).

Details zu Fibrinogen

Target: Fibrinogen

Abstract: Fibrinogen Produkte

Synonyme: fib2 antikoerper, MGC53620 antikoerper, fibrinogen antikoerper, sb:cb892 antikoerper, wu:fa55c12 antikoerper, wu:fb20e08 antikoerper, wu:fb59b03 antikoerper, wu:fi39d04 antikoerper, zgc:92117 antikoerper, fibrinogen alpha chain antikoerper, fibrinogen gamma chain antikoerper, fga antikoerper, FGA antikoerper, LOC698244 antikoerper, CpipJ_CPIJ010090 antikoerper, FGG antikoerper

Hintergrund: The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal rat plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products