Cholesterol Esterase Antikörper (Biotin)

Produktnummer ABIN459017

Produktdetails anti-Cholesterol Esterase Antikörper (Biotin)

Target: Cholesterol Esterase (CEL)

Reaktivität: Candida cylindracea

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cholesterol Esterase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Spezifität: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to cholesterol esterase from Candida cylindracea

Aufreinigung: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Cholesterol esterase isolated and purified from Candida cylindracea. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.5. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Details zu Cholesterol Esterase

Target: Cholesterol Esterase (CEL)

Andere Bezeichnung: Cholesterol Esterase (CEL Produkte)

Synonyme: SC8F4.24 antikoerper, BAL antikoerper, BSDL antikoerper, BSSL antikoerper, CELL antikoerper, CEase antikoerper, FAP antikoerper, FAPP antikoerper, LIPA antikoerper, MODY8 antikoerper, 1810036E18Rik antikoerper, Bal antikoerper, Bssl antikoerper, carboxyl ester lipase antikoerper, cholesterol esterase antikoerper, hypothetical protein antikoerper, CEL antikoerper, SCO5420 antikoerper, Tfu_2331 antikoerper, SARE_RS01070 antikoerper, RSal33209_1252 antikoerper, Caci_5976 antikoerper, Ndas_0054 antikoerper, Micau_5972 antikoerper, ML5_2523 antikoerper, Cel antikoerper, cel antikoerper

Hintergrund: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Pathways: Lipid Metabolism