Cholesterol Oxidase Antikörper (Biotin)

Produktnummer ABIN459018

Produktdetails anti-Cholesterol Oxidase Antikörper (Biotin)

Target: Cholesterol Oxidase (choS)

Reaktivität: Nocardia asteroides

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cholesterol Oxidase Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Spezifität: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to cholesterol oxidase from Nocardia erythropolis

Aufreinigung: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Cholesterol oxidase isolated and purified from Nocardia erythropolis. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 5.1. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is rec onstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Details zu Cholesterol Oxidase

Target: Cholesterol Oxidase (choS)

Andere Bezeichnung: Cholesterol Oxidase (choS Produkte)

Synonyme: SCD63.13 antikoerper, cholesterol oxidase antikoerper, GMC family oxidoreductase antikoerper, Cholesterol oxidase antikoerper, choS antikoerper, SCO4781 antikoerper, BTH_I0619 antikoerper, SARE_RS13770 antikoerper, Lcho_3191 antikoerper, Npun_R6526 antikoerper, Amir_2425 antikoerper, Caci_5961 antikoerper, Sros_4246 antikoerper, Kfla_6484 antikoerper, Snas_3535 antikoerper, Weevi_2091 antikoerper

Hintergrund: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.