Isocitrate Dehydrogenase Antikörper (Biotin)

Produktnummer ABIN459052

Produktdetails anti-Isocitrate Dehydrogenase Antikörper (Biotin)

Target: Isocitrate Dehydrogenase (IDH)

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Isocitrate Dehydrogenase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunofluorescence (IF), ELISA

Spezifität: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to isocitrate dehydrogenase from porcine heart

Aufreinigung: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Isocitrate dehydrogenase isolated and purified from porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.5. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Details zu Isocitrate Dehydrogenase

Target: Isocitrate Dehydrogenase (IDH)

Andere Bezeichnung: Iso Citrate Dehydrogenase (IDH Produkte)

Synonyme: CG7176 antikoerper, CT22171 antikoerper, Dmel\\CG7176 antikoerper, IDH antikoerper, IDH-NADP antikoerper, Idh-NADP antikoerper, idh antikoerper, isocitrate dehydrogenase antikoerper, l(3)L3852 antikoerper, F20D21.16 antikoerper, F20D21_16 antikoerper, SMU.672 antikoerper, icd antikoerper, Isocitrate dehydrogenase antikoerper, isocitrate dehydrogenase antikoerper, isocitrate dehydrogenase, NADP-dependent antikoerper, Monomeric isocitrate dehydrogenase antikoerper, ankyrin repeat domain 26-like antikoerper, Idh antikoerper, ICDH antikoerper, idh antikoerper, icd antikoerper, SSO_RS10660 antikoerper, JK_RS08640 antikoerper, LOC618625 antikoerper

Hintergrund: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.