Pyruvate Oxidase Antikörper (Biotin)

Produktnummer ABIN459105

Produktdetails anti-Pyruvate Oxidase Antikörper (Biotin)

Target: Pyruvate Oxidase (POX)

Reaktivität: Bakterien

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Pyruvate Oxidase Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Spezifität: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to pyruvate oxidase from bacteria

Aufreinigung: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Pyruvate oxidase isolated and purified from bacteria. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.0. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Details zu Pyruvate Oxidase

Target: Pyruvate Oxidase (POX)

Andere Bezeichnung: Pyruvate Oxidase (POX Produkte)

Synonyme: pyruvate oxidase antikoerper, thiamine pyrophosphate-containing protein YdaP antikoerper, hypothetical protein, similar to pyruvate oxidase antikoerper, similar to pyruvate oxidase antikoerper, SP_0730 antikoerper, spxB antikoerper, ydaP antikoerper, lmo0722 antikoerper, MW2460 antikoerper, pox1 antikoerper, pox5 antikoerper, SA2327 antikoerper, lin0730 antikoerper, PsycPRwf_1595 antikoerper, pox antikoerper

Hintergrund: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.