Collagenase Antikörper

Produktnummer ABIN459188

Produktdetails anti-Collagenase Antikörper

Target: Collagenase

Reaktivität: Clostridium histolyticum

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagenase Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Spezifität: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: IgG fraction of polyclonal rabbit antiserum to collagenase from Clostridium histolyticum

Aufreinigung: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Collagenase is isolated and purified from Clostridium histolyticum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 10mg lyof. in 1ml PBS

Buffer: Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Details zu Collagenase

Target: Collagenase

Abstract: Collagenase Produkte

Synonyme: collagenase antikoerper, RB3913 antikoerper, BPSS0666 antikoerper, Sbal_3732 antikoerper, Bcer98_0486 antikoerper, Shew185_0630 antikoerper, Shal_3056 antikoerper, Sbal195_0657 antikoerper, Lbys_3550 antikoerper, Palpr_2084 antikoerper

Hintergrund: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.