NADH Peroxidase Antikörper

Produktnummer ABIN459237

Produktdetails anti-NADH Peroxidase Antikörper

Target: NADH Peroxidase

Reaktivität: Enterococcus faecalis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NADH Peroxidase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), ELISA

Spezifität: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: IgG fraction of polyclonal rabbit antiserum to NADH peroxidase from Streptococcus faecalis

Aufreinigung: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: NADH peroxidase isolated and purified from Streptococcus faecalis. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG protein concentration 10 mg/ml. No foreign proteins added.

Buffer: Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Details zu NADH Peroxidase

Target: NADH Peroxidase

Abstract: NADH Peroxidase Produkte

Synonyme: putative NADH peroxidase antikoerper, NADH peroxidase antikoerper, NAD(FAD)-dependent dehydrogenase antikoerper, SPy_1681 antikoerper, spyM18_1693 antikoerper, npx antikoerper, SPs0401 antikoerper, npr antikoerper, MA_RS07405 antikoerper, M6_Spy1426 antikoerper, M5005_Spy_1378 antikoerper, M28_Spy1421 antikoerper, MBAR_RS14975 antikoerper, LSL_0137 antikoerper, MBUR_RS05050 antikoerper, SpyM50412 antikoerper, LCRIS_RS02695 antikoerper, Lbuc_1777 antikoerper

Hintergrund: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.