Pullulanase Antikörper
Reaktivität: Klebsiella aerogenes
IF, WB, ELISA
Wirt: Kaninchen
Polyclonal
unconjugated
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- Target Alle Pullulanase Produkte
- Pullulanase
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Reaktivität
- Klebsiella aerogenes
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Pullulanase Antikörper ist unkonjugiert
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Applikation
- Immunofluorescence (IF), Western Blotting (WB), ELISA
- Spezifität
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- Produktmerkmale
- IgG fraction of polyclonal rabbit antiserum to pullulanase from Enterobacter aerogenes
- Aufreinigung
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- Immunogen
- Pullulanase isolated and purified from Enterobacter aerogenes. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotyp
- IgG
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- Applikationshinweise
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Konzentration
- IgG protein concentration 10 mg/ml. No foreign proteins added.
- Buffer
- Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- Konservierungsmittel
- Without preservative
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Target
- Pullulanase
- Abstract
- Pullulanase Produkte
- Synonyme
- pullulanase antikoerper, TM1845 antikoerper, amyX antikoerper, Theth_0302 antikoerper
- Hintergrund
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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