FLIP Antikörper (AA 191-209)

Produktnummer ABIN461877

Produktdetails anti-FLIP Antikörper

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Bindungsspezifität: AA 191-209

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FLIP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Antibody recognizes human and murine FLIPgamma and FLIPdelta but not FLIPalpha and FLIPbeta.

Aufreinigung: Immunoaffinity purified

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC, IHC, IHC-P (5 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Can be used for detection of FLIP by Western blot at 1:1000 dilution. 35 kD and 25 kD bands can be detected. The antibody recognizes human and murine FLIPg and FLIPd but not FLIPa and FLIPb.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles. Store undiluted.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu FLIP

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Andere Bezeichnung: CFLAR / FLIP (CFLAR Produkte)

Hintergrund: Name/Gene ID: CFLAR
Subfamily: Cysteine C14
Family: Apoptosis

Synonyms: CFLAR, C-FLIPR, C-FLIPS, C-FLIP, CASP8AP1, Cflip, CLARP, CASH, Caspase homolog, Caspase-eight-related protein, Flice inhibitory protein, I-FLICE, Inhibitor of FLICE, FLAME, FLAME1, MRIT, Usurpin beta, C-FLIPL, Casper, FLAME-1, FLIP, Usurpin

Gen-ID: 8837

UniProt: O15519

Pathways: Apoptose, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development