Glutathione Peroxidase 1 Antikörper (AA 130-142)

Produktnummer ABIN461936

Produktdetails anti-Glutathione Peroxidase 1 Antikörper

Target: Glutathione Peroxidase 1 (GPX1)

Bindungsspezifität: AA 130-142

Reaktivität: Human, Affe, Pferd

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Glutathione Peroxidase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human GPX1. This antibody is expected to recognise isoform 1 ( NP_000572.2) only.

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Horse (100%) Panda, Bat, Pig (92%) Rat, Hamster, Zebu, Bovine, Dog, Rabbit (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-REALPAPSDDATA from an internal region of human GPX1 (NP_000572.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Horse (100%), Panda, Bat, Pig (92%), Rat, Hamster, Zebu, Bovine, Dog, Rabbit (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Glutathione Peroxidase 1

Target: Glutathione Peroxidase 1 (GPX1)

Andere Bezeichnung: GPX1 / Glutathione Peroxidase (GPX1 Produkte)

Hintergrund: Name/Gene ID: GPX1

Synonyms: GPX1, Glutathione peroxidase, GSHPx-1, Glutathione peroxidase 1, GPx-1, GSHPX1, GPXD

Gen-ID: 2876

NCBI Accession: NP_000572

UniProt: P07203

Pathways: Thyroid Hormone Synthesis, Sensory Perception of Sound, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interaktom