SRSF1 Antikörper (N-Term)

Produktnummer ABIN461956

Produktdetails anti-SRSF1 Antikörper

Target: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SRSF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Specific for the N-terminal portion (aa1-97) of the SF2/ASF protein comprising the RNA-recognition motif (RRM1). Reactivity is not affected by phosphorylation state of SF2/ASF. On Western blots, a band of ~33kD is seen with doublet or triplet bands sometimes evident due to phosphorylation differences. SF2/ASF is highly conserved (e. g. mouse and human protein are 100 % identical) so reactivity with other species is likely. Please note, however, that cross-reactivity in non-target species varies and cannot be guaranteed.

Aufreinigung: Protein A purified

Immunogen: Recombinant SF2/ASF protein.

Type of Immunogen: Recombinant protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IF, IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 1-3 μg/mL. A band of ~33kD is seen with doublet or triplet bands sometimes evident due to phosphorylation differences.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu SRSF1

Target: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Andere Bezeichnung: SFRS1 / SF2 (SRSF1 Produkte)

Hintergrund: Name/Gene ID: SRSF1

Synonyms: SRSF1, Alternative-splicing factor 1, ASF, SF2, SF2p33, SFRS1, Splicing factor 2, ASF-1, SR splicing factor 1, SRp30a

Gen-ID: 6426

UniProt: Q07955

Pathways: Ribonucleoprotein Complex Subunit Organization