BNIP3 Antikörper (AA 112-124)

Produktnummer ABIN461995

Produktdetails anti-BNIP3 Antikörper

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Bindungsspezifität: AA 112-124

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BNIP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Marke: IHC-plus™

Spezifität: Species cross-reactivity: Human.

Aufreinigung: Immunoaffinity purified

Immunogen: Recombinant fragment (Residues 1-163 of Human BNIP3). Epitope: The epitope recognized by the antibody resides within amino acids 112-124 of human BNIP3 molecule.

Type of Immunogen: Recombinant protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 2-4 μg/mL. Detects a band at ~30kD and ~60kD. Positive control: Whole cell lysate from 293T cells transfected with BNIP3.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 15 mM sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: 4°C while lyophilized, once reconstituted, aliquot and store at -70°C.

Details zu BNIP3

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Andere Bezeichnung: NIP3 / BNIP3 (BNIP3 Produkte)

Synonyme: NIP3 antikoerper, Nip3 antikoerper, BCL2 interacting protein 3 antikoerper, BCL2/adenovirus E1B interacting protein 3 antikoerper, BNIP3 antikoerper, Bnip3 antikoerper

Hintergrund: Name/Gene ID: BNIP3

Synonyms: BNIP3, NIP3

Gen-ID: 664

UniProt: Q12983

Pathways: Autophagie, Brown Fat Cell Differentiation