Caspase 3 Antikörper

Produktnummer ABIN462092

Produktdetails anti-Caspase 3 Antikörper

Target: Caspase 3 (CASP3)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Affe, Schwein, Kaninchen, Meerschweinchen, Hamster, Huhn, Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Aufreinigung: Protein A purified

Immunogen: Recombinant human Caspase-3 protein (full length).

Type of Immunogen: Recombinant protein

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % azide, 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Aliquot to Avoid freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Referenzen für anti-Caspase 3 Antikörper (ABIN462092)

Baumbach, Schrödl, Nenoff, Uhrlaß, Mülling, Michler: "Modeling dermatophytosis: Guinea pig skin explants represent a highly suitable model to study Trichophyton benhamiae infections." in: The Journal of dermatology, Vol. 47, Issue 1, pp. 8-16, (2020) (PubMed).

Domian, Surażyński, Szarmach, Piotrowska, Kasacka: "Mechanism of pro-apoptotic action of prosthetic restorations on oral mucosa cells." in: Advances in medical sciences, Vol. 65, Issue 1, pp. 134-140, (2020) (PubMed).

Details zu Caspase 3

Target: Caspase 3 (CASP3)

Andere Bezeichnung: CASP3 / Caspase 3 (CASP3 Produkte)

Hintergrund: Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama

Gen-ID: 836

UniProt: P42574

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation