Glucose-6-Phosphate Dehydrogenase Antikörper (AA 35-506)

Produktnummer ABIN462273

Produktdetails anti-Glucose-6-Phosphate Dehydrogenase Antikörper

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Bindungsspezifität: AA 35-506

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Glucose-6-Phosphate Dehydrogenase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human G6PD (35-506aa)

Aufreinigung: Protein G purified

Immunogen: Recombinant human G6PD (35-506aa) purified from E. coli

Type of Immunogen: Recombinant protein

Klon: AT2F6

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Cell lysates of MCF7 (30 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human G6PD (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Details zu Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Andere Bezeichnung: G6PD (G6PD Produkte)

Synonyme: G6PD1 antikoerper, g6pd antikoerper, G6pd antikoerper, G6pdx antikoerper, g6pdh antikoerper, g6pd2 antikoerper, fj78b06 antikoerper, wu:fj78b06 antikoerper, si:dkey-90a13.8 antikoerper, BA3433 antikoerper, CORTRD1 antikoerper, G6PDH antikoerper, GDH antikoerper, G28A antikoerper, G6PD antikoerper, Gpdx antikoerper, glucose-6-phosphate dehydrogenase antikoerper, glucose-6-phosphate 1-dehydrogenase antikoerper, glucose-6-phosphate 1-dehydrogenase (G6PD) antikoerper, glucose-6-P dehydrogenase antikoerper, glucose-6-phosphate dehydrogenase L homeolog antikoerper, glucose-6-phosphate 1-dehydrogenase Zwf antikoerper, hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase antikoerper, glucose-6-phosphate dehydrogenase X-linked antikoerper, G6PD antikoerper, g6pd antikoerper, g6pD antikoerper, zwf antikoerper, LOC100120232 antikoerper, LACBIDRAFT_188936 antikoerper, G6pd antikoerper, g6pd.L antikoerper, CNG03280 antikoerper, Tb10.70.5200 antikoerper, H6PD antikoerper, G6pdx antikoerper, H6pd antikoerper

Hintergrund: Name/Gene ID: G6PD

Synonyms: G6PD, G6PD1

Gen-ID: 2539

UniProt: P11413

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones