Caspase 7 Antikörper

Produktnummer ABIN462356

Produktdetails anti-Caspase 7 Antikörper

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Reaktivität: Human, Maus, Ratte, Affe, Schwein, Hund, Kaninchen, Hamster, Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Caspase 7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Detects an ~37 kD protein, corresponding to the apparent molecular mass of the caspase-7 on SDS-PAGE immunoblots, in samples from human, mouse, rat, monkey, dog, sheep, rabbit, pig and hamster origins. This antibody recognizes the unprocessed pro-caspase-7 (p35), caspase-7 lacking the short amino- terminal pro domain (32 kD) and the fully processed caspase-7 (p19) in apoptotic cell extracts.

Aufreinigung: Protein G purified

Immunogen: Recombinant human caspase-7 full length protein.

Type of Immunogen: Recombinant protein

Klon: 7-1-11

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS (10 mM sodium phosphate,  pH 7.2, 50 mM sodium chloride, 0.1 mM PMSF). Sourced in Ascites.

Konservierungsmittel: PMSF

Handhabung: avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Details zu Caspase 7

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Andere Bezeichnung: CASP7 / Caspase 7 (CASP7 Produkte)

Synonyme: xCaspase-7 antikoerper, caspase-7 antikoerper, caspase7 antikoerper, cmh-1 antikoerper, ice-lap3 antikoerper, mch3 antikoerper, CASP7 antikoerper, AI314680 antikoerper, CMH-1 antikoerper, ICE-IAP3 antikoerper, Mch3 antikoerper, mCASP-7 antikoerper, CASP-7 antikoerper, ICE-LAP3 antikoerper, LICE2 antikoerper, MCH3 antikoerper, Caspase-7 antikoerper, MCH3/SCA-2 antikoerper, SCA-2 antikoerper, caspase 7 antikoerper, caspase-7 antikoerper, caspase 7 S homeolog antikoerper, CASP7 antikoerper, xcaspase-7 antikoerper, casp7 antikoerper, casp7.S antikoerper, Casp7 antikoerper

Hintergrund: Name/Gene ID: CASP7
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP7, Apoptotic protease Mch-3, Caspase 7, Caspase-7, ICE-LAP3, LICE2, MCH3, ICE-like apoptotic protease 3, CASP-7, CMH-1

Gen-ID: 840

UniProt: P55210

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity