Cytochrome C Antikörper

Produktnummer ABIN462359

Produktdetails anti-Cytochrome C Antikörper

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reaktivität: Human, Ratte, Maus, Pferd, Kaninchen, Rind (Kuh), Schwein, Schaf, Affe, Hamster, Meerschweinchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytochrome C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Detects an ~14kD protein, corresponding to the apparent molecular mass of Cytochrome c on Western blots with samples from human, mouse, rat, bovine, guinea pig, hamster, monkey, rabbit and sheep origins. This antibody detects denatured Cytochrome c in applications such as Western blot, it does not recognize the native protein. The epitope of this antibody was mapped to a region within amino acids 93-104 of horse Cytochrome.

Aufreinigung: Protein G purified

Immunogen: Horse cytochrome c.

Type of Immunogen: Protein

Klon: 7H8-2C12

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Horse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 50 % glycerol, 0.09 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Andere Bezeichnung: CYCS / Cytochrome c (CYCS Produkte)

Hintergrund: Name/Gene ID: CYCS

Synonyms: CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c, somatic

Gen-ID: 54205

UniProt: P99999

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity