PF4 Antikörper
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- Target Alle PF4 Antikörper anzeigen
- PF4 (Platelet Factor 4 (PF4))
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser PF4 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA
- Spezifität
- Human PF-4
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human PF-4.
Type of Immunogen: Cell extract - Top Product
- Discover our top product PF4 Primärantikörper
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- Applikationshinweise
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Approved: ELISA (0.5 - 2 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 1 mg/mL-2 mg/mL with an overnight incubation at 4 °C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. ELISA: To detect hPF-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PF-4 (LS-C104446) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPF-4. Western Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Sterile water
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handhabung
- Avoid freeze/thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- PF4 (Platelet Factor 4 (PF4))
- Andere Bezeichnung
- CXCL4 / PF4 (PF4 Produkte)
- Synonyme
- CXCL4 antikoerper, PF-4 antikoerper, SCYB4 antikoerper, Cxcl4 antikoerper, Scyb4 antikoerper, Pf4a antikoerper, RATPF4A antikoerper, PF4A antikoerper, CXCL4L1 antikoerper, CXCL4V1 antikoerper, PF4-ALT antikoerper, SCYB4V1 antikoerper, PF4 antikoerper, platelet factor 4 antikoerper, platelet factor 4 variant 1 antikoerper, PF4 antikoerper, Pf4 antikoerper, LOC703451 antikoerper, PF4V1 antikoerper, LOC100524373 antikoerper, LOC102181854 antikoerper, LOC100630489 antikoerper
- Hintergrund
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Name/Gene ID: PF4
Subfamily: Intercrine alpha
Family: Intercrine
Synonyms: PF4, C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4, Platelet factor 4 - Gen-ID
- 5196
- UniProt
- P02776
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